iso ang 1 7 pd123319 pd123319  (MedChemExpress)

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Physical and chemical properties and biosafety assessment of Ang‐(1–7). (A) Key molecular properties: molecular weight (MW), net charge, isoelectric point (pI), hydrophobicity (GRAVY index), and aromaticity. (B, C) Topological structure predicted by Deep TMHMM, showing a spherical soluble conformation (no transmembrane helices) and predicted functional sites (red‐intracellular; blue‐extracellular). (D) Hemolytic activity ( n = 6). (E) Cytotoxicity assessment in H9c2, HepG2, and NRK‐52E cell lines ( n = 6). Cell viability was determined using the Cell Counting Kit‐8 (CCK‐8) assay. The data is expressed as an mean ± standard deviation (SD).

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Molecular Weight, Functional Assay, Activity Assay, Cell Counting, CCK-8 Assay, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Effects of Ang‐(1–7) on isoproterenol (ISO)‐induced cardiac hypertrophy and fibrosis. (A) HE staining and Masson staining of myocardial sections (scale bar = 20 μm). (B, C) Quantitative analysis of myocardial cell cross‐sectional area and collagen volume fraction. (D, E) Macroscopic morphology and cross‐sectional observation of the heart, with measurement of heart weight/body weight ratio (HW/BW) (scale bar = 2 mm). (F) Western blot analysis of ANP, BNP, and β‐MHC expression. (G–I) Quantitative analysis of band intensity normalized to GAPDH. The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM+A + I, A‐779 + Ang‐(1–7) + ISO; AntA+A + I, PD123319 + Ang‐(1–7) + ISO. *** p < 0.001 versus Ctrl; ### p < 0.001 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Staining, Western Blot, Expressing, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) improves ISO‐induced ventricular remodeling and dysfunction via MasR and AT 2 R. (A) Representative M‐mode echocardiograms from each group (scale bar = 2 mm). (B, C) Quantification of left ventricular internal diameters at systole (LVIDs) and diastole (LVIDd). (D, E) Left ventricular ejection fraction (LVEF%) and fractional shortening (LVFS%). (F, G) Left ventricular posterior wall thickness at systole (LVPWs) and diastole (LVPWd). (H) Representative immunofluorescence images of cardiomyocytes (green, α‐actinin; blue, nuclei; scale bar = 10 μm). (I) Relative mRNA expression of ANP, BNP, and β‐MHC ( n = 6). The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; AntA + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO. *** p < 0.001, ** p < 0.01 versus Ctrl; ### p < 0.001, ## p < 0.01 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO, & p < 0.05 versus ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Immunofluorescence, Expressing, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates the expression of MasR and AT 2 R in H9c2 cardiomyocytes and exhibits cross‐inhibition with receptor antagonists. (A) Immunofluorescence images and immunofluorescence intensity of MasR (green), AT 2 R (red), and DAPI (blue) in H9c2 cells from different treatment groups, reflecting receptor expression levels (scale bar = 80 μm) ( n = 5). (B) Shows the expression of AT 2 R and MasR mRNA in H9c2 cells ( n = 6). (C) Analyzes MasR expression and relative thermal stability in cardiomyocytes at different temperatures (37°C–58°C). The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; AntA + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO. *** p < 0.001, * p < 0.05 versus Ctrl; #### p < 0.0001, ### p < 0.001 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Expressing, Inhibition, Immunofluorescence, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates the expression of MasR and AT 2 R and their heterodimer formation. (A) Immunofluorescence staining images of MasR (green), AT 2 R (red), and DAPI (blue) in myocardial tissue from mice in each treatment group. Arrows indicate the localization of receptors in the myocardium (scale bar = 20 μm) ( n = 5). (B) Protein expression and quantitative analysis of MasR and AT 2 R in myocardial tissue ( n = 6). (C) Molecular docking simulations showing the interactions between MasR and AT 2 R, as well as between Ang‐(1–7) and the receptors, along with the binding energies (ΔG) for each interaction. (D) Immunoprecipitation (Co‐IP): Interaction between MasR and AT 2 R in cardiac muscle tissue. The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO. * p < 0.05 versus Ctrl; ### p < 0.001 versus ISO; &&& p < 0.001, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Expressing, Immunofluorescence, Staining, Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) attenuates ISO‐induced excessive autophagy and apoptosis in vivo by regulating MasR and AT 2 R. (A) Transmission electron microscopy (TEM) images showing autophagosomes, lysosomes, and damaged mitochondria in cardiomyocytes; quantification of damaged mitochondria is shown (scale bar = 5 μm, 2 μm n = 4). (B) Western blot analysis of autophagy‐related proteins LC3‐II/I, Beclin1, and P62 ( n = 5). (C) Western blot analysis of apoptosis‐related proteins Bcl‐2, Bax, and cleaved caspase‐3 ( n = 5). (D, E) Quantitative densitometry analysis of autophagy‐ and apoptosis‐related proteins normalized to GAPDH. The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO. *** p < 0.001 versus Ctrl; ### p < 0.001, # p < 0.05 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: In Vivo, Transmission Assay, Electron Microscopy, Western Blot, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates autophagic flux and apoptosis in H9c2 cardiomyocytes through MasR and AT 2 R. (A) Representative images of TUNEL immunofluorescence staining (green fluorescence labels apoptotic cells) and Hoechst nuclear staining (blue) ( n = 6), with the Merge panel showing their overlay. (B) Western blot analysis of apoptosis related proteins (Bax, Bcl 2 , Cleaved caspase‐3) expression levels, with GAPDH as the internal control protein ( n = 3). (C) Western blot analysis of autophagy‐related proteins (LC3‐II, Beclin1, P62) expression levels, with GAPDH as the internal control protein ( n = 3). The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: TUNEL Assay, Immunofluorescence, Staining, Fluorescence, Western Blot, Expressing, Control, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Physical and chemical properties and biosafety assessment of Ang‐(1–7). (A) Key molecular properties: molecular weight (MW), net charge, isoelectric point (pI), hydrophobicity (GRAVY index), and aromaticity. (B, C) Topological structure predicted by Deep TMHMM, showing a spherical soluble conformation (no transmembrane helices) and predicted functional sites (red‐intracellular; blue‐extracellular). (D) Hemolytic activity ( n = 6). (E) Cytotoxicity assessment in H9c2, HepG2, and NRK‐52E cell lines ( n = 6). Cell viability was determined using the Cell Counting Kit‐8 (CCK‐8) assay. The data is expressed as an mean ± standard deviation (SD).

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Molecular Weight, Functional Assay, Activity Assay, Cell Counting, CCK-8 Assay, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Effects of Ang‐(1–7) on isoproterenol (ISO)‐induced cardiac hypertrophy and fibrosis. (A) HE staining and Masson staining of myocardial sections (scale bar = 20 μm). (B, C) Quantitative analysis of myocardial cell cross‐sectional area and collagen volume fraction. (D, E) Macroscopic morphology and cross‐sectional observation of the heart, with measurement of heart weight/body weight ratio (HW/BW) (scale bar = 2 mm). (F) Western blot analysis of ANP, BNP, and β‐MHC expression. (G–I) Quantitative analysis of band intensity normalized to GAPDH. The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM+A + I, A‐779 + Ang‐(1–7) + ISO; AntA+A + I, PD123319 + Ang‐(1–7) + ISO. *** p < 0.001 versus Ctrl; ### p < 0.001 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Staining, Western Blot, Expressing, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) improves ISO‐induced ventricular remodeling and dysfunction via MasR and AT 2 R. (A) Representative M‐mode echocardiograms from each group (scale bar = 2 mm). (B, C) Quantification of left ventricular internal diameters at systole (LVIDs) and diastole (LVIDd). (D, E) Left ventricular ejection fraction (LVEF%) and fractional shortening (LVFS%). (F, G) Left ventricular posterior wall thickness at systole (LVPWs) and diastole (LVPWd). (H) Representative immunofluorescence images of cardiomyocytes (green, α‐actinin; blue, nuclei; scale bar = 10 μm). (I) Relative mRNA expression of ANP, BNP, and β‐MHC ( n = 6). The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; AntA + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO. *** p < 0.001, ** p < 0.01 versus Ctrl; ### p < 0.001, ## p < 0.01 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO, & p < 0.05 versus ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Immunofluorescence, Expressing, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates the expression of MasR and AT 2 R in H9c2 cardiomyocytes and exhibits cross‐inhibition with receptor antagonists. (A) Immunofluorescence images and immunofluorescence intensity of MasR (green), AT 2 R (red), and DAPI (blue) in H9c2 cells from different treatment groups, reflecting receptor expression levels (scale bar = 80 μm) ( n = 5). (B) Shows the expression of AT 2 R and MasR mRNA in H9c2 cells ( n = 6). (C) Analyzes MasR expression and relative thermal stability in cardiomyocytes at different temperatures (37°C–58°C). The data is expressed as an mean ± standard deviation (SD) ( n = 5). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; AntA + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO. *** p < 0.001, * p < 0.05 versus Ctrl; #### p < 0.0001, ### p < 0.001 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Expressing, Inhibition, Immunofluorescence, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates the expression of MasR and AT 2 R and their heterodimer formation. (A) Immunofluorescence staining images of MasR (green), AT 2 R (red), and DAPI (blue) in myocardial tissue from mice in each treatment group. Arrows indicate the localization of receptors in the myocardium (scale bar = 20 μm) ( n = 5). (B) Protein expression and quantitative analysis of MasR and AT 2 R in myocardial tissue ( n = 6). (C) Molecular docking simulations showing the interactions between MasR and AT 2 R, as well as between Ang‐(1–7) and the receptors, along with the binding energies (ΔG) for each interaction. (D) Immunoprecipitation (Co‐IP): Interaction between MasR and AT 2 R in cardiac muscle tissue. The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO. * p < 0.05 versus Ctrl; ### p < 0.001 versus ISO; &&& p < 0.001, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: Expressing, Immunofluorescence, Staining, Binding Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) attenuates ISO‐induced excessive autophagy and apoptosis in vivo by regulating MasR and AT 2 R. (A) Transmission electron microscopy (TEM) images showing autophagosomes, lysosomes, and damaged mitochondria in cardiomyocytes; quantification of damaged mitochondria is shown (scale bar = 5 μm, 2 μm n = 4). (B) Western blot analysis of autophagy‐related proteins LC3‐II/I, Beclin1, and P62 ( n = 5). (C) Western blot analysis of apoptosis‐related proteins Bcl‐2, Bax, and cleaved caspase‐3 ( n = 5). (D, E) Quantitative densitometry analysis of autophagy‐ and apoptosis‐related proteins normalized to GAPDH. The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO. *** p < 0.001 versus Ctrl; ### p < 0.001, # p < 0.05 versus ISO; &&& p < 0.001, && p < 0.01, & p < 0.05 versus Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: In Vivo, Transmission Assay, Electron Microscopy, Western Blot, Standard Deviation

Journal: Acta Physiologica (Oxford, England)

Article Title: Angiotensin‐(1–7) Alleviates Isoproterenol‐Induced Cardiac Hypertrophy by Suppressing Autophagy and Apoptosis Through the Synergistic Action of Mas Receptor and Angiotensin II Type 2 Receptor

doi: 10.1111/apha.70200

Figure Lengend Snippet: Ang‐(1–7) regulates autophagic flux and apoptosis in H9c2 cardiomyocytes through MasR and AT 2 R. (A) Representative images of TUNEL immunofluorescence staining (green fluorescence labels apoptotic cells) and Hoechst nuclear staining (blue) ( n = 6), with the Merge panel showing their overlay. (B) Western blot analysis of apoptosis related proteins (Bax, Bcl 2 , Cleaved caspase‐3) expression levels, with GAPDH as the internal control protein ( n = 3). (C) Western blot analysis of autophagy‐related proteins (LC3‐II, Beclin1, P62) expression levels, with GAPDH as the internal control protein ( n = 3). The data is expressed as an mean ± standard deviation (SD). A + I, Ang‐(1–7) + ISO; AntM + A + I, A‐779 + Ang‐(1–7) + ISO; Ant A + A + I, PD123319 + Ang‐(1–7) + ISO; AntM + AntA + A + I, A‐779 + PD123319 + Ang‐(1–7) + ISO.

Article Snippet: After 7 days' acclimatization, animals were randomized (blinded assessment) into five groups ( n = 6 per group) receiving daily Subcutaneous injection for 7 days [ , , ]:Ctrl: saline (Sanlian, Harbin, China); ISO: isoproterenol 5 mg/kg/day (Solarbio, II0200, Beijing, China; dissolved in DMSO); ISO + Ang‐(1–7): Ang‐(1–7) 576 μg/kg/day (MCE, HY‐12403, USA; in distilled water); ISO + Ang‐(1–7) + A‐779: A‐7791148 μg/kg/day (MCE, HY‐P0216, USA); ISO + Ang‐(1–7) + PD123319 : PD123319 5 mg/kg/day (MCE, HY‐10259A, USA); ISO + Ang‐(1–7) + PD123319 + A779.

Techniques: TUNEL Assay, Immunofluorescence, Staining, Fluorescence, Western Blot, Expressing, Control, Standard Deviation